ARG70340
Human K-Ras recombinant protein (His-tagged, N-ter)
Human K-Ras recombinant protein (His-tagged, N-ter) for SDS-PAGE and Human
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Human K-Ras recombinant protein. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | SDS-PAGE |
| Target Name | K-Ras |
| Species | Human |
| A.A. Sequence | Thr2 - Cys186 of Human K-Ras (NP_203524.1) with 6X His tag at the N-terminus. |
| Expression System | E. coli |
| Alternate Names | NS3; KRAS1; KRAS2; RALD; K-RAS4A; GTPase KRas; c-Ki-ras; KI-RAS; RASK2; K-RAS2B; K-RAS2A; K-RAS4B; c-K-ras; C-K-RAS; NS; CFC2; Ki-Ras; K-Ras 2 |
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 85% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRAS |
| Gene Full Name | Kirsten rat sarcoma viral oncogene homolog |
| Background | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] |
| Function | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed:24623306). [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. [UniProt] |
| Calculated MW | 22 kDa |
| PTM | Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). [UniProt] |
