ARG81264
Human Klotho ELISA Kit
Human Klotho ELISA Kit for ELISA and Human
Overview
| Product Description | ARG81264 Human Klotho ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Klotho in serum, plasma or cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Klotho |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 40 pg/ml |
| Sample Type | Serum, plasma or cell culture supernatants. |
| Standard Range | 39 - 2500 pg/ml |
| Sample Volume | 100 µl |
| Alternate Names | Klotho; EC 3.2.1.31 |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KL |
| Gene Full Name | klotho |
| Background | This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008] |
| Function | May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. [UniProt] |
| Highlight | Related products: Klotho antibodies; Klotho ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. |
| Title | Download Link |
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| ARG81264 Human Klotho ELISA Kit User's manual |
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