ARG81866

Human LBP ELISA Kit

Human LBP ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81866-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81866-002 Standard 2 X 50 ng/vial 4°C
ARG81866-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81866-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81866-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81866-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81866-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81866-008 25X Wash buffer 20 ml 4°C
ARG81866-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81866-010 STOP solution 10 ml (Ready to use) 4°C
ARG81866-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81866 Human LBP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LBP in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name LBP
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%; Inter-Assay CV: 6.3%
Alternate Names LBP; Lipopolysaccharide-binding protein; BPIFD2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3929 Human LBP

Swiss-port # P18428 Human Lipopolysaccharide-binding protein

Gene Symbol LBP
Gene Full Name lipopolysaccharide binding protein
Background The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP). [provided by RefSeq, Apr 2012]
Function Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide. [UniProt]
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Title Download Link
ARG81866 Human LBP ELISA Kit User's manual download icon Download