ARG81952

Human LDL receptor ELISA Kit

Human LDL receptor ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81952-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81952-002 Standard 2 X 10 ng/vial 4°C
ARG81952-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81952-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81952-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81952-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81952-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81952-008 25X Wash buffer 20 ml 4°C
ARG81952-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81952-010 STOP solution 10 ml (Ready to use) 4°C
ARG81952-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81952 Human LDL receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LDL receptor in serum, plasma (heparin), urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name LDL Receptor
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin), urine and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%; Inter-Assay CV: 6.6%
Alternate Names FH; LDLCQ2; Low-density lipoprotein receptor; LDL receptor; FHC

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3949 Human LDLR

Swiss-port # P01130 Human Low-density lipoprotein receptor

Gene Symbol LDLR
Gene Full Name low density lipoprotein receptor
Background The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
Function Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. [UniProt]
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LDL Receptor antibodies; LDL Receptor ELISA Kits;
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PTM N- and O-glycosylated.

Ubiquitinated by MYLIP leading to degradation. [UniProt]
Title Download Link
ARG81952 Human LDL receptor ELISA Kit User's manual download icon Download