ARG70245
Human LIF recombinant protein (Active) (His-tagged, C-ter)
Human LIF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Active Human LIF recombinant protein. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FuncSt, SDS-PAGE |
| Target Name | LIF |
| Species | Human |
| A.A. Sequence | Ser23 - Phe202 of Human LIF (NP_002300.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng/ml. |
| Alternate Names | LIF; Leukemia inhibitory factor; Emfilermin; CDF; DIA; Differentiation-stimulating factor; MLPLI; HILDA; Melanoma-derived LPL inhibitor; D factor |
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LIF |
| Gene Full Name | leukemia inhibitory factor |
| Background | The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Function | LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 22 kDa |
