Component

Overview

Product Description	ARG81568 Human LOXL2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human LOXL2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity	Hu
Tested Application	ELISA
Specificity	There is no detectable cross-reactivity with other relevant proteins.
Target Name	LOXL2
Conjugation	HRP
Conjugation Note	Substrate: TMB and read at 450 nm.
Sensitivity	78 pg/ml
Sample Type	Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range	156 - 10000 pg/ml
Sample Volume	100 µl
Precision	Intra-Assay CV: 6.4% Inter-Assay CV: 7.0%
Alternate Names	Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14; LOR2; Lysyl oxidase-like protein 2; Lysyl oxidase homolog 2; EC 1.4.3.13; WS9-14

Application Instructions

Assay Time	~ 5 hours

Properties

Form	96 well
Storage Instruction	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 4017 Human LOXL2 Swiss-port # Q9Y4K0 Human Lysyl oxidase homolog 2
Gene Symbol	LOXL2
Gene Full Name	lysyl oxidase-like 2
Background	This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]
Function	Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. [UniProt]
Highlight	Related products: LOXL2 antibodies; LOXL2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData
PTM	The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. N-glycosylated. N-glycosylation on Asn-455 and Asn-644 may be essential for proper folding and secretion; may be composed of a fucosylated carbohydrates attached to a trimannose N-linked glycan core. [UniProt]