ARG82700

Human Legumain (total) ELISA Kit

Human Legumain (total) ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82700-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82700-002 Standard 2 X 10 ng/vial 4°C
ARG82700-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82700-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82700-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82700-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82700-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82700-008 25X Wash buffer 20 ml 4°C
ARG82700-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82700-010 STOP solution 10 ml (Ready to use) 4°C
ARG82700-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82700 Human Legumain (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Legumain (total) in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Legumain (total)
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 16 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.4%
Inter-Assay CV: 7.1%
Alternate Names PRSC1; AEP; Protease, cysteine 1; LGMN1; Asparaginyl endopeptidase; Legumain; EC 3.4.22.34

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5641 Human LGMN

Swiss-port # Q99538 Human Legumain

Gene Symbol LGMN
Gene Full Name legumain
Background This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]
Function Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. [UniProt]
Cellular Localization Lysosome. [UniProt]
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PTM Glycosylated.

Activated by autocatalytic processing at pH 4. [UniProt]
Title Download Link
ARG82700 Human Legumain (total) ELISA Kit User manual download icon Download