ARG82580
Human Leptin ELISA Kit
Human Leptin ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82580-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82580-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82580-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82580-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82580-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82580-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82580-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82580-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82580-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82580-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82580-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82580 Human Leptin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Leptin in Serum, Plasma (EDTA. Heparin) , Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Leptin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5% Inter-Assay CV: 7.8% |
| Alternate Names | Leptin; Obese protein; OB; LEPD; Obesity factor; OBS |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | LEP |
|---|---|
| Gene Full Name | leptin |
| Background | This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008] |
| Function | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. [UniProt] |
| Highlight | Related products: Leptin antibodies; Leptin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Neuroscience kit; Signaling Transduction kit |
| Title | Download Link |
|---|---|
| ARG82580 Human Leptin ELISA kit User manual |
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