ARG81616

Human Lp-PLA2 ELISA Kit

Human Lp-PLA2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81616-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81616-002 Standard 2 X 50 ng/vial 4°C
ARG81616-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81616-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81616-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81616-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81616-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81616-008 25X Wash buffer 20 ml 4°C
ARG81616-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81616-010 STOP solution 10 ml (Ready to use) 4°C
ARG81616-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81616 Human Lp-PLA2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Lp-PLA2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Lp-PLA2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.0%
Inter-Assay CV: 6.5%
Alternate Names LDL-PLA; gVIIA-PLA2; LDL-PLA2; Group-VIIA phospholipase A2; 2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1.47; LDL-associated phospholipase A2; PAF 2-acylhydrolase; 2; PAF acetylhydrolase; PAFAH; Platelet-activating factor acetylhydrolase; PAFAD; 1-alkyl-2-acetylglycerophosphocholine esterase; LP-PLA2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7941 Human PLA2G7

Swiss-port # Q13093 Human Platelet-activating factor acetylhydrolase

Gene Symbol PLA2G7
Gene Full Name phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
Background The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2009]
Function Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. [UniProt]
Highlight Related products:
Lp-PLA2 antibodies; Lp-PLA2 ELISA Kits; Lp-PLA2 Duos / Panels;
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Title Download Link
ARG81616 Human Lp-PLA2 ELISA Kit User's manual download icon Download