ARG82546

Human MAG ELISA Kit

Human MAG ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82546-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82546-002 Standard 2 X 10 ng/vial 4°C
ARG82546-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82546-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82546-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82546-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82546-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82546-008 25X Wash buffer 20 ml 4°C
ARG82546-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82546-010 STOP solution 10 ml (Ready to use) 4°C
ARG82546-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82546 Human MAG ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MAG in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name MAG
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 156 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 312 - 20000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.9%
Inter-Assay CV: 6.1%
Alternate Names GMA; Myelin-associated glycoprotein; SIGLEC4A; S-MAG; Siglec-4a; SIGLEC-4A

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4099 Human MAG

Swiss-port # P20916 Human Myelin-associated glycoprotein

Gene Symbol MAG
Gene Full Name myelin associated glycoprotein
Background The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]
Function Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Membrane raft. [UniProt]
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PTM N-glycosylated.

Phosphorylated on tyrosine residues. [UniProt]
Title Download Link
ARG82546 Human MAG ELISA Kit User manual download icon Download