ARG82705
Human MAdCAM1 ELISA Kit
Human MAdCAM1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82705-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82705-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82705-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82705-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82705-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82705-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82705-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82705-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82705-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82705-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82705-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82705 Human MAdCAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MAdCAM1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | MAdCAM1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 16 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.5% Inter-Assay CV: 7.0% |
| Alternate Names | Mucosal addressin cell adhesion molecule 1; hMAdCAM-1; MAdCAM-1; MACAM1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13477 Human Mucosal addressin cell adhesion molecule 1 |
|---|---|
| Gene Symbol | MADCAM1 |
| Gene Full Name | mucosal vascular addressin cell adhesion molecule 1 |
| Background | The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: MAdCAM1 antibodies; MAdCAM1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82705 Human MAdCAM1 ELISA kit User manual |
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