ARG81789

Human MICB ELISA Kit

Human MICB ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81789-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81789-002 Standard 2 X 10 ng/vial 4°C
ARG81789-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81789-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81789-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81789-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81789-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81789-008 25X Wash buffer 20 ml 4°C
ARG81789-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81789-010 STOP solution 10 ml (Ready to use) 4°C
ARG81789-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81789 Human MICB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MICB in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MICB
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%; Inter-Assay CV: 6.7%
Alternate Names MHC class I polypeptide-related sequence B; MIC-B; PERB11.2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4277 Human MICB

Swiss-port # Q29980 Human MHC class I polypeptide-related sequence B

Gene Symbol MICB
Gene Full Name MHC class I polypeptide-related sequence B
Background This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt]
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MICB antibodies; MICB ELISA Kits;
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PTM Proteolytically cleaved and released from the cell surface of tumor cells. [UniProt]
Title Download Link
ARG81789 Human MICB ELISA Kit User's manual download icon Download