ARG81294

Human MIF ELISA Kit

Human MIF ELISA Kit for ELISA and Human

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Overview

Product Description

ARG81294 Human MIF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MIF in serum, plasma (platelet free) and cell culture supernatants

Tested Reactivity Hu
Tested Application ELISA
Target Name MIF
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 15 pg/ml
Sample Type serum, plasma (platelet free) and cell culture supernatants
Standard Range 31.25 - 2000 pg/ml
Sample Volume 100 µl
Alternate Names Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12

Application Instructions

Assay Time ~ 3.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4282 Human MIF

Swiss-port # P14174 Human Macrophage migration inhibitory factor

Gene Symbol MIF
Gene Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Background This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
Function Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. [UniProt]
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MIF antibodies; MIF ELISA Kits; MIF Duos / Panels;
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Simplify the ELISA analysis by GainData

Specific References

An ultrasensitive polydopamine bi-functionalized SERS immunoassay for exosome-based diagnosis and classification of pancreatic cancer.

ELISA / Human

Li Teng-Da et al.
Chemical Science.,  (2018)

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