ARG70276

Human MMP1 recombinant protein (Active) (His-tagged, C-ter)

Human MMP1 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Active Human MMP1 recombinant protein.
Tested Reactivity Hu
Tested Application FuncSt, SDS-PAGE
Target Name MMP1
Species Human
A.A. Sequence Phe20 - Asn469 of Human MMP1 (NP_002412.1) with 6X His tag at the C-terminus.
Expression System HEK293
Activity Active
Activity Note Measured in a cell migration assay using A549 cells. 1 ng/ml of Recombinant Human MMP-1 can effectively induce A549 cells migration.
Alternate Names MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 97% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4312 Human MMP1

Swiss-port # P03956 Human Interstitial collagenase

Gene Symbol MMP1
Gene Full Name matrix metallopeptidase 1
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:2557822, PubMed:2153297, PubMed:1645757). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369). [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 54 kDa
PTM Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Tyrosine phosphorylated in platelets by PKDCC/VLK. [UniProt]