ARG81488

Human MMP12 ELISA Kit

Human MMP12 ELISA Kit for ELISA and Human

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Component

Cat No Component Name Package Temp
ARG81488-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81488-002 Standard 2 X 10 ng/vial 4°C
ARG81488-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81488-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81488-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81488-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81488-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81488-008 25X Wash buffer 20 ml 4°C
ARG81488-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81488-010 STOP solution 10 ml (Ready to use) 4°C
ARG81488-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81488 Human MMP12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MMP12 in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MMP12
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 6.2%
Alternate Names ME; EC 3.4.24.65; Macrophage metalloelastase; MMP-12; hME; MME; HME; Matrix metalloproteinase-12; Macrophage elastase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4321 Human MMP12

Swiss-port # P39900 Human Macrophage metalloelastase

Gene Symbol MMP12
Gene Full Name matrix metallopeptidase 12
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
Function May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. [UniProt]
Highlight Related products:
MMP12 antibodies; MMP12 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Specific References

Adipose-derived stem cells enriched with therapeutic mRNA TGF-β3 and IL-10 synergistically promote scar-less wound healing in preclinical models

ELISA / Human / Culture supernatant

Wei Wang et al.
Bioengineering & Translational Medicine,  (2023)

publication_link

 

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ARG81488 Human MMP12 ELISA Kit User's manual download icon Download