ARG70280

Human MMP13 recombinant protein (Active) (His-tagged, C-ter)

Human MMP13 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Active Human MMP13 recombinant protein.
Tested Reactivity Hu
Tested Application FuncSt, SDS-PAGE
Target Name MMP13
Species Human
A.A. Sequence Leu20 - Cys471 of Human MMP13 (NP_002418.1) with 6X His tag at the C-terminus.
Expression System HEK293
Activity Active
Activity Note Measured in a cell migration assay using A549 cells. 10 ng/ml of Recombinant Human MMP-13 can effectively induce A549 cells migration.
Alternate Names CLG3; EC 3.4.24.-; MANDP1; MMP-13; Collagenase 3; Matrix metalloproteinase-13

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4322 Human MMP13

Swiss-port # P45452 Human Collagenase 3

Gene Symbol MMP13
Gene Full Name matrix metallopeptidase 13
Background This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]
Function Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. Secreted. [UniProt]
Calculated MW 54 kDa
PTM The proenzyme is activated by removal of the propeptide; this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro).

N-glycosylated.

Tyrosine phosphorylated by PKDCC/VLK. [UniProt]