ARG82633
Human MMP14 / MT1-MMP Assay Kit
Human MMP14 / MT1-MMP Assay Kit for Functional study and Human
Overview
| Product Description | ARG82633 Human MMP14 / MT1-MMP Assay Kit is a detection kit for the quantification of Human MMP14 in cell and tissue lysates. |
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| Tested Reactivity | Hu |
| Tested Application | FuncSt |
| Specificity | Measures endogenous active MMP14 (naturally occurring). |
| Target Name | MMP14 / MT1-MMP |
| Conjugation Note | Read at 405 nm. |
| Sensitivity | 500 pg/ml for 2 hours incubation 100 pg/ml for 5 hours incubation |
| Sample Type | Cell and tissue lysates |
| Standard Range | 100 - 16000 pg/ml |
| Sample Volume | 100 µl |
| Alternate Names | MT1MMP; MT-MMP 1; Membrane-type matrix metalloproteinase 1; MT1-MMP; Membrane-type-1 matrix metalloproteinase; MT-MMP; EC 3.4.24.80; MMP-X1; MMP-14; Matrix metalloproteinase-14; WNCHRS; MTMMP1 |
Application Instructions
| Assay Time | Overnight |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP14 |
| Gene Full Name | matrix metallopeptidase 14 (membrane-inserted) |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008] |
| Function | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. [UniProt] |
| PTM | The precursor is cleaved by a furin endopeptidase. Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
| Title | Download Link |
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| ARG82633 Human MMP14 Assay kit User manual |
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