ARG82633

Human MMP14 / MT1-MMP Assay Kit

Human MMP14 / MT1-MMP Assay Kit for Functional study and Human

Overview

Product Description ARG82633 Human MMP14 / MT1-MMP Assay Kit is a detection kit for the quantification of Human MMP14 in cell and tissue lysates.
Tested Reactivity Hu
Tested Application FuncSt
Specificity Measures endogenous active MMP14 (naturally occurring).
Target Name MMP14 / MT1-MMP
Conjugation Note Read at 405 nm.
Sensitivity 500 pg/ml for 2 hours incubation
100 pg/ml for 5 hours incubation
Sample Type Cell and tissue lysates
Standard Range 100 - 16000 pg/ml
Sample Volume 100 µl
Alternate Names MT1MMP; MT-MMP 1; Membrane-type matrix metalloproteinase 1; MT1-MMP; Membrane-type-1 matrix metalloproteinase; MT-MMP; EC 3.4.24.80; MMP-X1; MMP-14; Matrix metalloproteinase-14; WNCHRS; MTMMP1

Application Instructions

Assay Time Overnight

Properties

Form 96 well
Storage Instruction Store the kit at -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4323 Human MMP14

Swiss-port # P50281 Human Matrix metalloproteinase-14

Gene Symbol MMP14
Gene Full Name matrix metallopeptidase 14 (membrane-inserted)
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
Function Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm. [UniProt]
PTM The precursor is cleaved by a furin endopeptidase.

Tyrosine phosphorylated by PKDCC/VLK. [UniProt]
Title Download Link
ARG82633 Human MMP14 Assay kit User manual download icon Download