ARG82631
Human MMP9 Assay Kit
Human MMP9 Assay Kit for Functional study and Human
Overview
| Product Description | ARG82631 Human MMP9 Assay Kit is a detection kit for the quantification of Human MMP9 in serum, plasma, cell culture supernatants, urine, synovial fluid and tissue homogenates. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FuncSt |
| Specificity | Measures endogenous active MMP9 (naturally occurring) or total active MMP9 (following activation with APMA). |
| Target Name | MMP9 |
| Conjugation Note | Read at 405 nm. |
| Sensitivity | 500 pg/ml for 1 hour incubation 10 pg/ml for 4 hours incubation |
| Detection Range | 10 - 16000 pg/ml |
| Sample Type | Serum, plasma, cell culture supernatants, urine, synovial fluid and tissue homogenates. |
| Sample Volume | 100 µl |
| Alternate Names | Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase |
Application Instructions
| Assay Time | Overnight |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MMP9 |
| Gene Full Name | matrix metallopeptidase 9 |
| Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008] |
| Function | May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. [UniProt] |
| PTM | Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated. [UniProt] |
| Title | Download Link |
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| ARG82631 Human MMP9 Assay kit User manual |
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