ARG82631

Human MMP9 Assay Kit

Human MMP9 Assay Kit for Functional study and Human

Overview

Product Description ARG82631 Human MMP9 Assay Kit is a detection kit for the quantification of Human MMP9 in serum, plasma, cell culture supernatants, urine, synovial fluid and tissue homogenates.
Tested Reactivity Hu
Tested Application FuncSt
Specificity Measures endogenous active MMP9 (naturally occurring) or total active MMP9 (following activation with APMA).
Target Name MMP9
Conjugation Note Read at 405 nm.
Sensitivity 500 pg/ml for 1 hour incubation
10 pg/ml for 4 hours incubation
Detection Range 10 - 16000 pg/ml
Sample Type Serum, plasma, cell culture supernatants, urine, synovial fluid and tissue homogenates.
Sample Volume 100 µl
Alternate Names Matrix metalloproteinase-9; 92 kDa gelatinase; MMP-9; Gelatinase B; GELB; CLG4B; MANDP2; EC 3.4.24.35; 92 kDa type IV collagenase

Application Instructions

Assay Time Overnight

Properties

Form 96 well
Storage Instruction Store the kit at -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4318 Human MMP9

Swiss-port # P14780 Human Matrix metalloproteinase-9

Gene Symbol MMP9
Gene Full Name matrix metallopeptidase 9
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
PTM Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.

N- and O-glycosylated. [UniProt]
Title Download Link
ARG82631 Human MMP9 Assay kit User manual download icon Download