ARG82620

Human MUC16 / CA125 ELISA Kit

Human MUC16 / CA125 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82620-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82620-002 Standard 2 X 10 ng/vial 4°C
ARG82620-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82620-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82620-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82620-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82620-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82620-008 25X Wash buffer 20 ml 4°C
ARG82620-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82620-010 STOP solution 10 ml (Ready to use) 4°C
ARG82620-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82620 Human MUC16 / CA125 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MUC16 / CA125 in Human serum, plasma (EDTA, heparin), cell culture supernatants, saliva, urine and milk.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MUC16 / CA125
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 10 pg/ml
Sample Type Serum, plasma (EDTA, heparin), cell culture supernatants, saliva, urine and milk.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 6.2%
Alternate Names Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 94025 Human MUC16

Swiss-port # Q8WXI7 Human Mucin-16

Gene Symbol MUC16
Gene Full Name mucin 16, cell surface associated
Function Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. [UniProt]
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PTM Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.

Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.

May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.

May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. [UniProt]
Title Download Link
ARG82620 Human MUC16 / CA125 ELISA Kit User manual download icon Download