ARG70268
Human NCR3 / NKp30 recombinant protein (ECD) (Fc-His-tagged, C-ter)
Human NCR3 / NKp30 recombinant protein (ECD) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) Human NCR3 / NKp30 recombinant protein (ECD). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | NCR3 / NKp30 (ECD) |
| Species | Human |
| A.A. Sequence | Leu19 - Thr138 of Human NCR3 / NKp30 (NP_001138938.1) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | NK-p30; 1C7; CD antigen CD337; Activating natural killer receptor p30; NKp30; Natural cytotoxicity triggering receptor 3; MALS; LY117; Natural killer cell p30-related protein; CD337 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant Human B7-H6 at 2µg/ml (100 µl/well) can bind recombinant Human NCR3, the EC50 of Human NCR3 is 66.43 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O14931 Human Natural cytotoxicity triggering receptor 3 |
|---|---|
| Gene Symbol | NCR3 |
| Gene Full Name | natural cytotoxicity triggering receptor 3 |
| Background | The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010] |
| Function | Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 22 kDa |
