ARG81602

Human NECTIN4 / PVRL4 ELISA Kit

Human NECTIN4 / PVRL4 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81602-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81602-002 Standard 2 X 10 ng/vial 4°C
ARG81602-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81602-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81602-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81602-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81602-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81602-008 25X Wash buffer 20 ml 4°C
ARG81602-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81602-010 STOP solution 10 ml (Ready to use) 4°C
ARG81602-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81602 Human NECTIN4 / PVRL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NECTIN4 / PVRL4 in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name NECTIN4 / PVRL4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 6.1%
Alternate Names Nectin-4; Ig superfamily receptor LNIR; PRR4; LNIR; nectin-4; EDSS1; Poliovirus receptor-related protein 4; Nectin cell adhesion molecule 4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81607 Human PVRL4

Swiss-port # Q96NY8 Human Nectin-4

Gene Symbol PVRL4
Gene Full Name poliovirus receptor-related 4
Background This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]
Function Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254). Processed poliovirus receptor-related protein 4: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625). [UniProt]
Highlight New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. [UniProt]
Title Download Link
ARG81602 Human PVRL4 ELISA Kit User's manual download icon Download