ARG80941

Human NGAL / Lipocalin-2 ELISA Kit

Human NGAL / Lipocalin-2 ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit; Metabolism kit; Signaling Transduction kit

Overview

Product Description

ARG80941 Human NGAL / Lipocalin-2 ELISA Kit is a Enzyme Immunoassay kit for the quantification of Human NGAL / Lipocalin-2 in serum, plasma and cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Target Name Lipocalin 2
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm.

Sensitivity 39 pg/ml
Sample Type Serum, plasma and cell culture supernatants
Standard Range 78 - 5000 pg/ml
Sample Volume 100 μl
Alternate Names Siderocalin LCN2; Oncogene 24p3; MSFI; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; p25; Neutrophil gelatinase-associated lipocalin; 24p3; NGAL

Application Instructions

Assay Time ~200 min

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3934 Human LCN2

Swiss-port # P80188 Human Neutrophil gelatinase-associated lipocalin

Gene Symbol LCN2
Gene Full Name lipocalin 2
Background This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]
Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. [UniProt]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit; Metabolism kit; Signaling Transduction kit
Title Download Link
ARG80941 Human NGAL / Lipocalin-2 ELISA Kit User's manual download icon Download