ARG80941
Human NGAL / Lipocalin-2 ELISA Kit
Human NGAL / Lipocalin-2 ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit; Metabolism kit; Signaling Transduction kit
Overview
| Product Description | ARG80941 Human NGAL / Lipocalin-2 ELISA Kit is a Enzyme Immunoassay kit for the quantification of Human NGAL / Lipocalin-2 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Lipocalin 2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 39 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 μl |
| Alternate Names | Siderocalin LCN2; Oncogene 24p3; MSFI; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; p25; Neutrophil gelatinase-associated lipocalin; 24p3; NGAL |
Application Instructions
| Assay Time | ~200 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P80188 Human Neutrophil gelatinase-associated lipocalin |
|---|---|
| Gene Symbol | LCN2 |
| Gene Full Name | lipocalin 2 |
| Background | This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015] |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. [UniProt] |
| Highlight | Related products: NGAL antibodies; NGAL ELISA Kits; Related news: A new role of fat – factory of circulating miRNAs New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Cell Death kit; Immune System kit; Metabolism kit; Signaling Transduction kit |
| Title | Download Link |
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| ARG80941 Human NGAL / Lipocalin-2 ELISA Kit User's manual |
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