ARG82440

Human Nectin 3 ELISA Kit

Human Nectin 3 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82440-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82440-002 Standard 2 X 10 ng/vial 4°C
ARG82440-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82440-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82440-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82440-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82440-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82440-008 25X Wash buffer 20 ml 4°C
ARG82440-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82440-010 STOP solution 10 ml (Ready to use) 4°C
ARG82440-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82440 Human Nectin 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Nectin 3 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Nectin 3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 62.5 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 125 - 8000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%
Inter-Assay CV: 5.3%
Alternate Names PVRR3; PPR3; CDW113; PRR3; Nectin-3; CDw113; CD113; NECTIN-3; Poliovirus receptor-related protein 3; CD antigen CD113

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25945 Human PVRL3

Swiss-port # Q9NQS3 Human Nectin-3

Gene Symbol PVRL3
Gene Full Name poliovirus receptor-related 3
Background This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Function Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. [UniProt]
Cellular Localization Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. [UniProt]
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Title Download Link
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