ARG81508

Human Nesfatin 1 ELISA Kit

Human Nesfatin 1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81508-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81508-002 Standard 2 X 10 ng/vial 4°C
ARG81508-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81508-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81508-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81508-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81508-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81508-008 25X Wash buffer 20 ml 4°C
ARG81508-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81508-010 STOP solution 10 ml (Ready to use) 4°C
ARG81508-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81508 Human Nesfatin 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Nesfatin 1 in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Nesfatin 1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 6.6%
Alternate Names HEL-S-109; NEFA; Nucleobindin-2; Gastric cancer antigen Zg4; Prepronesfatin; DNA-binding protein NEFA

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4925 Human NUCB2

Swiss-port # P80303 Human Nucleobindin-2

Gene Symbol NUCB2
Gene Full Name nucleobindin 2
Background This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]
Function Calcium-binding protein. May have a role in calcium homeostasis.

Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity). [UniProt]
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Title Download Link
ARG81508 Human Nesfatin 1 ELISA Kit User's manual download icon Download