ARG80186
Human Neuroserpin ELISA Kit
Human Neuroserpin ELISA Kit for ELISA and Human
Neuroscience kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80186-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80186-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80186-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80186-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80186-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80186-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80186-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80186-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80186-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80186-010 | STOP solution | 12ml | 4°C |
| ARG80186-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80186 Human Neuroserpin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Neuroserpin in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-2,3,4,5,6,7,9,10,12, IFN- gamma, TNF- alpha；mouse neuroserpin |
| Target Name | Neuroserpin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 30 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Peptidase inhibitor 12; neuroserpin; Neuroserpin; Serpin I1; PI12; PI-12 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPINI1 |
| Gene Full Name | serpin peptidase inhibitor, clade I (neuroserpin), member 1 |
| Background | Neuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological target of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degradation of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. In the adult brain, neuroserpin is secreted from the growth cones of neurons in areas where synaptic changes are associated with learning and memory, i.e. cerebral cortex, hippocampus, and amygdala. The neuroprotective role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin, caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with neuroserpin inclusion bodies (FENIB). Recombinant human neuroserpin is a 44.6 kDa non-glycosylated protein containing 394 amino-acid residues. |
| Highlight | Related products: Neuroserpin antibodies; Neuroserpin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Neuroscience kit |
| Title | Download Link |
|---|---|
| ARG80186 Human Neuroserpin ELISA Kit User's manual |
 Download
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