ARG70144

Human Neurturin recombinant protein (Active) (His-tagged, C-ter)

Human Neurturin recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

Overview

Product Description E. coli expressed, His-tagged (C-ter) Active Human Neurturin recombinant protein
Tested Application SDS-PAGE
Target Name Neurturin
Species Human
A.A. Sequence MARLG ARPCG LRELE VRVSE LGLGY ASDET VLFRY CAGAC EAAAR VYDLG LRRLR QRRRL RRERV RAQPC CRPTA YEDEV SFLDA HSRYH TVHEL SAREC ACV with polyhistidine tag at the C-terminus.
Expression System E. coli
Activity Active
Activity Note Determined by its ability to induce proliferation in SH-SY5Y cells. The ED50 for this effect is < 50 ng/ml.
Alternate Names Neurturin; NTN

Properties

Form Powder
Purification Ni-NTA chromatography.
Purification Note Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 98% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
Storage Instruction Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol NRTN
Gene Full Name neurturin
Background Neurturin is a member of the TGF-beta subfamily, TRN. This gene signals through RET and a GPI-linked coreceptor, and promotes survival of neuronal populations. A neurturin mutation has been described in a family with Hirschsprung Disease. [provided by RefSeq, Jul 2008]
Function Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. [UniProt]
Cellular Localization Secreted. [UniProt]