ARG82775
Human Neutrophil Elastase ELISA Kit
Human Neutrophil Elastase ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82775-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82775-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82775-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82775-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82775-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82775-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82775-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82775-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82775-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82775-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82775-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82775 Human Neutrophil Elastase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Neutrophil Elastase in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Neutrophil Elastase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.3% Inter-Assay CV: 5.3% |
| Alternate Names | Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ELANE |
| Gene Full Name | elastase, neutrophil expressed |
| Background | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016] |
| Function | Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). [UniProt] |
| Title | Download Link |
|---|---|
| ARG82775 Human Neutrophil Elastase ELISA Kit User manual |
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