ARG82775

Human Neutrophil Elastase ELISA Kit

Human Neutrophil Elastase ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82775-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82775-002 Standard 2 X 50 ng/vial 4°C
ARG82775-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82775-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82775-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82775-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82775-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82775-008 25X Wash buffer 20 ml 4°C
ARG82775-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82775-010 STOP solution 10 ml (Ready to use) 4°C
ARG82775-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82775 Human Neutrophil Elastase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Neutrophil Elastase in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Neutrophil Elastase
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.3%
Inter-Assay CV: 5.3%
Alternate Names Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1991 Human ELANE

Swiss-port # P08246 Human Neutrophil elastase

Gene Symbol ELANE
Gene Full Name elastase, neutrophil expressed
Background Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]
Function Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). [UniProt]
Title Download Link
ARG82775 Human Neutrophil Elastase ELISA Kit User manual download icon Download