ARG81575

Human NrCAM ELISA Kit

Human NrCAM ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81575-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81575-002 Standard 2 X 10 ng/vial 4°C
ARG81575-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81575-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81575-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81575-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81575-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81575-008 25X Wash buffer 20 ml 4°C
ARG81575-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81575-010 STOP solution 10 ml (Ready to use) 4°C
ARG81575-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81575 Human NrCAM ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NrCAM in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name NrCAM
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 6.1%
Alternate Names Nr-CAM; Ng-CAM-related; hBravo; Neuronal surface protein Bravo; Neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4897 Human NRCAM

Swiss-port # Q92823 Human Neuronal cell adhesion molecule

Gene Symbol NRCAM
Gene Full Name neuronal cell adhesion molecule
Background Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier (By similarity). [UniProt]
Highlight Related products:
NrCAM antibodies; NrCAM ELISA Kits;
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Simplify the ELISA analysis by GainData
Title Download Link
ARG81575 Human NrCAM ELISA Kit User's manual download icon Download