ARG81418
Human Osteoprotegerin ELISA Kit
Human Osteoprotegerin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81418-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81418-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81418-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81418-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81418-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81418-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81418-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81418-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81418-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81418-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81418-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81418 Human Osteoprotegerin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Osteoprotegerin in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Osteoprotegerin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.85 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9% Inter-Assay CV: 7.1% |
| Alternate Names | OCIF; Osteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B; PDB5; Osteoprotegerin; OPG; TR1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00300 Human Tumor necrosis factor receptor superfamily member 11B |
|---|---|
| Gene Symbol | TNFRSF11B |
| Gene Full Name | tumor necrosis factor receptor superfamily, member 11b |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. [UniProt] |
| Highlight | Related products: Osteoprotegerin antibodies; Osteoprotegerin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. Contains sialic acid residues. The N-terminus is blocked. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81418 Human Osteoprotegerin ELISA Kit User's manual |
 Download
|
