ARG82639

Human PDGF AA ELISA Kit

Human PDGF AA ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82639-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82639-002 Standard 2 X 10 ng/vial 4°C
ARG82639-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82639-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82639-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82639-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82639-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82639-008 25X Wash buffer 20 ml 4°C
ARG82639-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82639-010 STOP solution 10 ml (Ready to use) 4°C
ARG82639-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82639 Human PDGF AA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PDGF AA in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name PDGF AA
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.0%
Inter-Assay CV: 5.5%
Alternate Names Platelet-derived growth factor subunit A; PDGF subunit A; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; PDGF-1; PDGF1; PDGF-A

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5154 Human PDGFA

Swiss-port # P04085 Human Platelet-derived growth factor subunit A

Gene Symbol PDGFA
Gene Full Name platelet-derived growth factor alpha polypeptide
Background This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. [UniProt]
Cellular Localization Secreted. Note=Released by platelets upon wounding. [UniProt]
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Title Download Link
ARG82639 Human PDGF AA ELISA Kit User manual download icon Download