ARG81400

Human PDGF AB ELISA Kit

Human PDGF AB ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81400-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81400-002 Standard 2 X 10 ng/vial 4°C
ARG81400-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81400-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81400-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81400-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81400-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81400-008 25X Wash buffer 20 ml 4°C
ARG81400-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81400-010 STOP solution 10 ml (Ready to use) 4°C
ARG81400-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81400 Human PDGF AB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PDGF AB in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name PDGF AB
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.5%
Inter-Assay CV: 6.7%
Alternate Names Platelet-derived growth factor subunit A; PDGF subunit A; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; PDGF-1; PDGF1; PDGF-A; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5154 Human PDGFA

Swiss-port # P04085 Human Platelet-derived growth factor subunit A

Gene Symbol PDGFA; PDGFB
Gene Full Name platelet derived growth factor subunit A; platelet derived growth factor subunit B
Background The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity). [UniProt]
Highlight Related products:
PDGF antibodies; PDGF ELISA Kits;
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Title Download Link
ARG81400 Human PDGF AB ELISA Kit User's manual download icon Download