ARG82058

Human PDGF DD ELISA Kit

Human PDGF DD ELISA Kit for ELISA and Human

Overview

Product Description Human PDGF DD ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PDGF DD in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name PDGF DD
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Alternate Names SCDGFB; Iris-expressed growth factor; PDGFD receptor-binding form; PDGFD latent form; MSTP036; Spinal cord-derived growth factor B; IEGF; PDGF-D; SCDGF-B; Platelet-derived growth factor D

Application Instructions

Assay Time 4.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 80310 Human PDGFD

Swiss-port # Q9GZP0 Human Platelet-derived growth factor D

Gene Symbol PDGFD
Gene Full Name platelet derived growth factor D
Background The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix (By similarity). [UniProt]
Cellular Localization Secreted. Note=Released by platelets upon wounding. [UniProt]
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PTM Activated by proteolytic cleavage. Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after Arg-247 or Arg-249 by urokinase plasminogen activator gives rise to the active form. [UniProt]
Title Download Link
ARG82058 Human PDGF DD ELISA Kit User manual download icon Download