ARG82834

Human PLA2G4A ELISA Kit

Human PLA2G4A ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82834-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82834-002 Standard 2 X 10 ng/vial 4°C
ARG82834-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82834-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82834-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82834-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82834-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82834-008 25X Wash buffer 20 ml 4°C
ARG82834-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82834-010 STOP solution 10 ml (Ready to use) 4°C
ARG82834-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82834 Human PLA2G4A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PLA2G4A in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name PLA2G4A
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.3 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.8%
Inter-Assay CV: 6.6%
Alternate Names Phospholipase A2 group IVA; cPLA2; cPLA2-alpha; Phosphatidylcholine 2-acylhydrolase; PLA2G4; Cytosolic phospholipase A2; EC 3.1.1.5; EC 3.1.1.4

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5321 Human PLA2G4A

Swiss-port # P47712 Human Cytosolic phospholipase A2

Gene Symbol PLA2G4A
Gene Full Name phospholipase A2, group IVA (cytosolic, calcium-dependent)
Background This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Function Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. [UniProt]
Cellular Localization Cytoplasm. Cytoplasmic vesicle. Note=Translocates to membrane vesicles in a calcium-dependent fashion. [UniProt]
PTM Activated by phosphorylation at both Ser-505 and Ser-727. [UniProt]
Title Download Link
ARG82834 Human PLA2G4A ELISA Kit User manual download icon Download