ARG82754

Human PRELP ELISA Kit

Human PRELP ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82754-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82754-002 Standard 2 X 10 ng/vial 4°C
ARG82754-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82754-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82754-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82754-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82754-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82754-008 25X Wash buffer 20 ml 4°C
ARG82754-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82754-010 STOP solution 10 ml (Ready to use) 4°C
ARG82754-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82754 Human PRELP ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PRELP in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name PRELP
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 50 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 93.8 - 6000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%
Inter-Assay CV: 6.3%
Alternate Names MST161; SLRR2A; Prolargin; Proline-arginine-rich end leucine-rich repeat protein; MSTP161

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5549 Human PRELP

Swiss-port # P51888 Human Prolargin

Gene Symbol PRELP
Gene Full Name proline/arginine-rich end leucine-rich repeat protein
Background The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Function May anchor basement membranes to the underlying connective tissue. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
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Title Download Link
ARG82754 Human PRELP ELISA kit User manual download icon Download