ARG81429

Human PROK1 / EG-VEGF ELISA Kit

Human PROK1 / EG-VEGF ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81429-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81429-002 Standard 2 X 10 ng/vial 4°C
ARG81429-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81429-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81429-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81429-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81429-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81429-008 25X Wash buffer 20 ml 4°C
ARG81429-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81429-010 STOP solution 10 ml (Ready to use) 4°C
ARG81429-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81429 Human PROK1 / EG-VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PROK1 / EG-VEGF in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name PROK1 / EG-VEGF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%
Inter-Assay CV: 7.2%
Alternate Names PK1; Endocrine-gland-derived vascular endothelial growth factor; Prokineticin-1; PRK1; EG-VEGF; Mambakine; EGVEGF

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 84432 Human PROK1

Swiss-port # P58294 Human Prokineticin-1

Gene Symbol PROK1
Gene Full Name prokineticin 1
Background The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]
Function Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. [UniProt]
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Title Download Link
ARG81429 Human PROK1 / EG-VEGF ELISA Kit User's manual download icon Download