ARG82566

Human PTHR1 ELISA Kit

Human PTHR1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82566-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82566-002 Standard 2 X 10 ng/vial 4°C
ARG82566-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82566-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82566-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82566-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82566-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82566-008 25X Wash buffer 20 ml 4°C
ARG82566-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82566-010 STOP solution 10 ml (Ready to use) 4°C
ARG82566-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82566 Human PTHR1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PTHR1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name PTHR1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 5.6%
Alternate Names Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH/PTHrP type I receptor; Parathyroid hormone 1 receptor; PTHR1; PTH/PTHr receptor; PFE; PTHR; PTH1 receptor

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5745 Human PTH1R

Swiss-port # Q03431 Human Parathyroid hormone/parathyroid hormone-related peptide receptor

Gene Symbol PTH1R
Gene Full Name parathyroid hormone 1 receptor
Background The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]
Function This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. [UniProt]
Cellular Localization Cell membrane; Multi-pass membrane protein. [UniProt]
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Title Download Link
ARG82566 Human PTHR1 ELISA Kit User manual download icon Download