ARG82714
Human PZP / Pregnancy zone protein ELISA Kit
Human PZP / Pregnancy zone protein ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82714-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82714-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82714-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82714-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82714-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82714-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82714-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82714-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82714-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82714-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82714-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82714 Human PZP / Pregnancy zone protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PZP / Pregnancy zone protein in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | PZP / Pregnancy zone protein |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 6.4% |
| Alternate Names | CPAMD6 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PZP |
| Gene Full Name | PZP alpha-2-macroglobulin like |
| Background | The protein encoded by this gene is highly expressed in late-pregnancy serum and is similar in structure to alpha-2-macroglobulin. The encoded protein, which acts as a homotetramer, inhibits the activity of all four classes of proteinases. This protein contains cleavage sites for several proteinases. Upon binding of a proteinase, the conformation of this protein changes to trap the proteinase, limiting its activity. This protein appears to be elevated in the sera of presymptomatic Alzheimer's disease patients. [provided by RefSeq, Dec 2016] |
| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [UniProt] |
| Highlight | Related products: PZP antibodies; PZP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82714 Human PZP / Pregnancy zone protein ELISA kit User manual |
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