ARG82393

Human Plexin B2 ELISA Kit

Human Plexin B2 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82393-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82393-002 Standard 2 X 40 ng/vial 4°C
ARG82393-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82393-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82393-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82393-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82393-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82393-008 25X Wash buffer 20 ml 4°C
ARG82393-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82393-010 STOP solution 10 ml (Ready to use) 4°C
ARG82393-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82393 Human Plexin B2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Plexin B2 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name Plexin B2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.312 ng/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 0.625 - 40 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%
Inter-Assay CV: 7.4%
Alternate Names Plexin-B2; Nbla00445; MM1; PLEXB2; dJ402G11.3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23654 Human PLXNB2

Swiss-port # O15031 Human Plexin-B2

Gene Symbol PLXNB2
Gene Full Name plexin B2
Background Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins (Perrot et al. (2002) [PubMed 12183458]).[supplied by OMIM, Mar 2008]
Function Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation and migration of neuronal cells during brain corticogenesis and for normal embryonic brain development. Regulates the migration of cerebellar granule cells in the developing brain. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. May modulate the activity of RAC1 and CDC42. Down-regulates macrophage migration in wound-healing assays (in vitro) (By similarity). [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
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Title Download Link
ARG82393 Human Plexin B2 ELISA Kit User manual download icon Download