ARG81493

Human Progranulin ELISA Kit

Human Progranulin ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81493-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81493-002 Standard 2 X 100 ng/vial 4°C
ARG81493-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81493-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81493-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81493-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81493-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81493-008 25X Wash buffer 20 ml 4°C
ARG81493-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81493-010 STOP solution 10 ml (Ready to use) 4°C
ARG81493-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81493 Human Progranulin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Progranulin in serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Progranulin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.78 ng/ml
Sample Type Serum, plasma (heparin, EDTA), saliva, urine, human milk and cell culture supernatants.
Standard Range 1.56 - 100 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.8%
Inter-Assay CV: 7.7%
Alternate Names PEPI; Glycoprotein 88; CLN11; GEP; Proepithelin; Granulin E; Granulin D; Granulins; PGRN; Glycoprotein of 88 Kda; Granulin G; Progranulin; PCDGF; Granulin B; Granulin C; Granulin A; GP88; Granulin F

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2896 Human GRN

Swiss-port # P28799 Human Granulins

Gene Symbol GRN
Gene Full Name granulin
Background Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]
Function Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.

Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. [UniProt]
Highlight Related products:
Progranulin antibodies; Progranulin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Granulins are disulfide bridged. [UniProt]
Title Download Link
ARG81493 Human Progranulin ELISA Kit User's manual download icon Download