ARG81117
Human Prothrombin (total) ELISA Kit
Human Prothrombin (total) ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit
Overview
| Product Description | ARG81117 Human Prothrombin (total) ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Prothrombin (total) in plasma. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Prothrombin |
| Conjugation | HRP |
| Conjugation Note | TMB substrate is used for color development at 450 nm. |
| Sensitivity | 0.166 ng/ml |
| Sample Type | Plasma |
| Standard Range | 0.25 - 100 ng/ml |
| Alternate Names | PT; EC 3.4.21.5; Prothrombin; THPH1; Coagulation factor II; RPRGL2 |
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F2 |
| Gene Full Name | coagulation factor II (thrombin) |
| Background | Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
| Function | Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. [UniProt] |
| Highlight | Related products: Thrombin antibodies; Thrombin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit |
| PTM | The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). |
| Title | Download Link |
|---|---|
| ARG81117 Human Prothrombin (total) ELISA Kit User's manual |
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