ARG81754

Human RANK ELISA Kit

Human RANK ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81754-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81754-002 Standard 2 X 10 ng/vial 4°C
ARG81754-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81754-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81754-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81754-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81754-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81754-008 25X Wash buffer 20 ml 4°C
ARG81754-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81754-010 STOP solution 10 ml (Ready to use) 4°C
ARG81754-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81754 Human RANK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RANK in serum and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name RANK
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.2%; Inter-Assay CV: 6.5%
Alternate Names ODFR; Osteoclast differentiation factor receptor; OFE; FEO; CD265; PDB2; CD antigen CD265; RANK; Receptor activator of NF-KB; OPTB7; Tumor necrosis factor receptor superfamily member 11A; TRANCER; LOH18CR1; OSTS

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8792 Human TNFRSF11A

Swiss-port # Q9Y6Q6 Human Tumor necrosis factor receptor superfamily member 11A

Gene Symbol TNFRSF11A
Gene Full Name tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]
Function Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. [UniProt]
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RANK antibodies; RANK ELISA Kits;
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Title Download Link
ARG81754 Human RANK ELISA Kit User's manual download icon Download