ARG80155
Human RANTES ELISA Kit
Human RANTES ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Immune System kit
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80155-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80155-002 | Standard (Lyophilized) | 3 X 0.5 ng/vial | 4°C |
| ARG80155-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80155-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80155-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80155-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80155-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80155-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80155-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80155-010 | STOP solution | 12ml | 4°C |
| ARG80155-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80155 Human RANTES ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RANTES in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human GRO alpha, IL-8, MCP-1, MIP-1 alpha, MIP-1 beta；mouse MIP-1 alpha, MIP-1 beta |
| Target Name | RANTES |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 1.95 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 3.9 - 250 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | 3-68; C-C motif chemokine 5; SIS-delta; D17S136E; SISd; T cell-specific protein P228; EoCP; Small-inducible cytokine A5; Eosinophil chemotactic cytokine; TCP228; 4-68; RANTES; SCYA5; eoCP; T-cell-specific protein RANTES |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL5 |
| Gene Full Name | chemokine (C-C motif) ligand 5 |
| Background | This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. [provided by RefSeq] |
| Highlight | Related products: RANTES antibodies; RANTES ELISA Kits; Related news: circNDUFB2, a circular RNA (circRNA), activates anti-tumor immunity New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Immune System kit |
| PTM | N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells. The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. |
Specific References
circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity.
ELISA / Human / Culture medium
| Title | Download Link |
|---|---|
| ARG80155 Human RANTES ELISA Kit User's manual |
 Download
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