ARG80155

Human RANTES ELISA Kit

Human RANTES ELISA Kit for ELISA and Human

Cell Biology and Cellular Response kit; Immune System kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80155-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80155-002 Standard (Lyophilized) 3 X 0.5 ng/vial 4°C
ARG80155-003 Standard diluent buffer 20 ml 4°C
ARG80155-004 Antibody conjugate concentrate 400 µl 4°C
ARG80155-005 Antibody diluent buffer 16 ml 4°C
ARG80155-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80155-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80155-008 20X Wash buffer 50 ml 4°C
ARG80155-009 TMB substrate 12ml 4°C (Protect from light)
ARG80155-010 STOP solution 12ml 4°C
ARG80155-011 Plate sealer 4 strips Room temperature

Overview

Product Description

ARG80155 Human RANTES ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RANTES in Serum, Plasma, Cell culture supernatants.

Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human GRO alpha, IL-8, MCP-1, MIP-1 alpha, MIP-1 beta;mouse MIP-1 alpha, MIP-1 beta
Target Name RANTES
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 1.95 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 3.9 - 250 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names 3-68; C-C motif chemokine 5; SIS-delta; D17S136E; SISd; T cell-specific protein P228; EoCP; Small-inducible cytokine A5; Eosinophil chemotactic cytokine; TCP228; 4-68; RANTES; SCYA5; eoCP; T-cell-specific protein RANTES

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6352 Human CCL5

Swiss-port # P13501 Human C-C motif chemokine 5

Gene Symbol CCL5
Gene Full Name chemokine (C-C motif) ligand 5
Background This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. [provided by RefSeq]
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Research Area Cell Biology and Cellular Response kit; Immune System kit
PTM N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.

Specific References

circNDUFB2 inhibits non-small cell lung cancer progression via destabilizing IGF2BPs and activating anti-tumor immunity.

ELISA / Human / Culture medium

Botai Li et al.
Nat Commun.,  (2021)

publication_link

 

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