ARG82390

Human REG1 alpha ELISA Kit

Human REG1 alpha ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82390-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82390-002 Standard 2 X 10 ng/vial 4°C
ARG82390-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82390-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82390-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82390-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82390-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82390-008 25X Wash buffer 20 ml 4°C
ARG82390-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82390-010 STOP solution 10 ml (Ready to use) 4°C
ARG82390-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82390 Human REG1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human REG1 alpha in serum, plasma (heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name REG1 alpha
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%
Inter-Assay CV: 6.3%
Alternate Names Islet cells regeneration factor; Regenerating protein I alpha; Lithostathine-1-alpha; Islet of Langerhans regenerating protein; PSPS1; ICRF; REG-1-alpha; Regenerating islet-derived protein 1-alpha; P19; PSP; Pancreatic thread protein; PSPS; REG; Pancreatic stone protein; PTP

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5967 Human REG1A

Swiss-port # P05451 Human Lithostathine-1-alpha

Gene Symbol REG1A
Gene Full Name regenerating islet-derived 1 alpha
Background This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008]
Function Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. [UniProt]
Cellular Localization Secreted. [UniProt]
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PTM The composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine. [UniProt]
Title Download Link
ARG82390 Human REG1 alpha ELISA Kit User manual download icon Download