ARG81597

Human RSPO1 ELISA Kit

Human RSPO1 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81597-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81597-002 Standard 2 X 10 ng/vial 4°C
ARG81597-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81597-004 Antibody conjugate concentrate (100X) 1 vial (120 µl) 4°C
ARG81597-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81597-006 HRP-Streptavidin concentrate (100X) 1 vial (120 µl) 4°C
ARG81597-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81597-008 20X Wash buffer 30 ml 4°C
ARG81597-009 TMB substrate 12 ml (Ready to use) 4°C (Protect from light)
ARG81597-010 STOP solution 12 ml (Ready to use) 4°C
ARG81597-011 Plate sealer 3 strips Room temperature

Overview

Product Description ARG81597 Human RSPO1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RSPO1 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name RSPO1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 5.9%
Alternate Names hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 284654 Human RSPO1

Swiss-port # Q2MKA7 Human R-spondin-1

Gene Symbol RSPO1
Gene Full Name R-spondin 1
Background This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Function Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. [UniProt]
Highlight Related products:
RSPO1 antibodies; RSPO1 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Title Download Link
ARG81597 Human RSPO1 ELISA Kit User's manual download icon Download