ARG70238
Human RSPO1 / R spondin 1 recombinant protein (Active) (His-tagged, C-ter)
Human RSPO1 / R spondin 1 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE,Functional study and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Active Human RSPO1 / R spondin 1 recombinant protein. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FuncSt, SDS-PAGE |
| Target Name | RSPO1 / R spondin 1 |
| Species | Human |
| A.A. Sequence | Arg31 - Ala263 of Human RSPO1 / R spondin 1 (NP_001033722.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Activity | Active |
| Activity Note | Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10 ng/ml of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression. |
| Alternate Names | hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3 |
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 92% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RSPO1 |
| Gene Full Name | R-spondin 1 |
| Background | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed:17804805). [UniProt] |
| Cellular Localization | Secreted. Nucleus. Note=Seems to mainly localize to nucleoli. [UniProt] |
| Calculated MW | 29 kDa |
