ARG81589

Human RSPO4 ELISA Kit

Human RSPO4 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81589-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81589-002 Standard 2 X 10 ng/vial 4°C
ARG81589-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81589-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81589-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81589-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81589-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81589-008 25X Wash buffer 20 ml 4°C
ARG81589-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81589-010 STOP solution 10 ml (Ready to use) 4°C
ARG81589-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81589 Human RSPO4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RSPO4 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name RSPO4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.8%
Inter-Assay CV: 6.6%
Alternate Names CRISTIN4; Roof plate-specific spondin-4; hRspo4; R-spondin-4; C20orf182

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 343637 Human RSPO4

Swiss-port # Q2I0M5 Human R-spondin-4

Gene Symbol RSPO4
Gene Full Name R-spondin 4
Background This gene encodes a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]
Function Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. [UniProt]
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PTM Tyr-112 may be phosphorylated; however as this position is probably extracellular, the vivo relevance is not proven. [UniProt]
Title Download Link
ARG81589 Human RSPO4 ELISA Kit User's manual download icon Download