ARG81516

Human S100A12 ELISA Kit

Human S100A12 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81516-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81516-002 Standard 2 X 10 ng/vial 4°C
ARG81516-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81516-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81516-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81516-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81516-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81516-008 25X Wash buffer 20 ml 4°C
ARG81516-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81516-010 STOP solution 10 ml (Ready to use) 4°C
ARG81516-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81516 Human S100A12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human S100A12 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name S100A12
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.3%
Inter-Assay CV: 7.7%
Alternate Names Calgranulin-C; S100 calcium-binding protein A12; p6; Neutrophil S100 protein; Calcium-binding protein in amniotic fluid 1; MRP-6; ENRAGE; Migration inhibitory factor-related protein 6; CAAF1; EN-RAGE; Protein S100-A12; CAGC; Extracellular newly identified RAGE-binding protein; CGRP; MRP6

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6283 Human S100A12

Swiss-port # P80511 Human Protein S100-A12

Gene Symbol S100A12
Gene Full Name S100 calcium binding protein A12
Background The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]
Function S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. [UniProt]
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Title Download Link
ARG81516 Human S100A12 ELISA Kit User's manual download icon Download