ARG80159
Human SCF ELISA Kit
Human SCF ELISA Kit for ELISA and Human
Developmental Biology kit; Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80159-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80159-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80159-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80159-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80159-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80159-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80159-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80159-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80159-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80159-010 | STOP solution | 12ml | 4°C |
| ARG80159-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80159 Human SCF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SCF in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human CNTF, EGF, Epo, G-CSF, GM-CSF, IL-1 alpha, IL-1 beta,2,3,4,5,6,7,8, LIF, SCFR, TGF- beta1, TNF- alpha |
| Target Name | SCF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | SCF; SHEP7; MGF; FPHH; sKITLG; Stem cell factor; KL-1; Kitl; Kit ligand; Mast cell growth factor; c-Kit ligand; FPH2; SF |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KITLG |
| Gene Full Name | KIT ligand |
| Background | Stem Cell Factor (SCF), also called c-kit Ligand or Mast Cell Growth Factor (MGF), is a peptide growth factor/cytokine with broad activities, especially related to hematopoiesis (ref 1-3). SCF acts on early hematopoietic progenitor/stem cells and stimulates the proliferation and survival of mast cells. Also, SCF is one of the most potent stimulators of multi-lineage progenitors (CFU-GEMM) in both human and murine bone marrow cells (ref 2,4). SCF acts synergistically with other growth factors, including erythropoietin, G-CSF, M-CSF, GM-CSF, IL-3 and IL-6, to increase the number and size of colonies of hematopoietic progenitors (ref 1,2,5). SCF appears to play an important role in the survival, proliferation or migration of primordial germ cells and melanoblasts during both development and maturation (ref 6,7). |
| Highlight | Related products: SCF antibodies; SCF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Developmental Biology kit; Immune System kit |
| PTM | A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. A soluble form exists as a cleavage product of the extracellular domain. |
| Title | Download Link |
|---|---|
| ARG80159 Human SCF ELISA Kit User's manual |
 Download
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