ARG81626
Human SERPING1 / C1 Inhibitor ELISA Kit
Human SERPING1 / C1 Inhibitor ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81626-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81626-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81626-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81626-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81626-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81626-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81626-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81626-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81626-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81626-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81626-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81626 Human SERPING1 / C1 Inhibitor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SERPING1 / C1 Inhibitor in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | SERPING1 / C1 Inhibitor |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.8% |
| Alternate Names | C1IN; C1INH; C1-inhibiting factor; Serpin G1; C1 esterase inhibitor; Plasma protease C1 inhibitor; C1 Inh; HAE2; C1Inh; C1NH; HAE1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPING1 |
| Gene Full Name | serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 |
| Background | This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq, Jul 2008] |
| Function | Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein. [UniProt] |
| Highlight | Related products: Serpin antibodies; Serpin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Highly glycosylated (49%) with N- and O-glycosylation. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan heterogeneity at Asn-25: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor). Can be proteolytically cleaved by E.coli stcE. [UniProt] |
| Title | Download Link |
|---|---|
| ARG81626 Human SERPING1 / C1 Inhibitor ELISA Kit User's manual |
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