ARG82533
Human SPINK5 ELISA Kit
Human SPINK5 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82533-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82533-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82533-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82533-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82533-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82533-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82533-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82533-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82533-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82533-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82533-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82533 Human SPINK5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SPINK5 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | SPINK5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 5.8% |
| Alternate Names | LETKI; LEKTI; Lympho-epithelial Kazal-type-related inhibitor; Serine protease inhibitor Kazal-type 5; VAKTI; NS; NETS |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NQ38 Human Serine protease inhibitor Kazal-type 5 |
|---|---|
| Gene Symbol | SPINK5 |
| Gene Full Name | serine peptidase inhibitor, Kazal type 5 |
| Background | This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Proteolytically processed by furin in individual domains (D1, D5, D6, D8 through D11, and D9 through D15) exhibiting various inhibitory potentials for multiple proteases. [UniProt] |
| Title | Download Link |
|---|---|
| ARG82533 Human SPINK5 ELISA Kit User manual |
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